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Published on: June 2, 2017
Comparative Evaluation of Bactericidal Effect of Silver Nanoparticle in Combination with Nd-YAG Laser against
Nandhini Ambalavanan1, Mahendran Kavitha2, Srilekha Jayakumar1
1Department of Conservative Dentistry and Endodontics, Tamil Nadu Government Dental College and Hospital, The Tamil Nadu Dr MGR Medical University, Chennai, Tamil Nadu, India.
Aim And Objective:
To evaluate the bactericidal effect of silver nanoparticles and silver nanoparticles in combination with Nd-YAG laser against Enterococcus faecalis.
Materials And Methods:
A solution containing 100 μg of silver nanoparticles in 1 mL was prepared by adding 5 mL of 10-4 M solution of AgNO3 with 5 mL of 0.1 M sodium tricitrate. Synthesized silver nanoparticles were characterized using UV-visible spectrophotometer for optical studies and the transmission electron microscopic analysis for determining the size and shape of the nanoparticles. Groups are as follows: group I-silver nanoparticles against E. faecalis, group II-silver nanoparticles in combination with Nd-YAG laser against E. faecalis, group III-control, E. faecalis bacterial culture alone. Optical density was measured periodically at half an hour interval in spectrophotometer in a 96 well plate and statistically analyzed using one-way ANOVA.
Results:
The optical density and turbidity of groups I and II began to decrease in 2 hours in comparison with the control. There was a significant difference in mean optical density among the three groups after 1½ hours onward. The study also demonstrated the minimal bactericidal concentration (MBC) as 100 μg/mL of Ag nanoparticles with a size of 15 nm were effective against E. faecalis.
Conclusion:
The study concluded that silver nanoparticles individually and in conjunction with Nd:YAG laser irradiation would be an effective protocol against E. faecalis.
Clinical Significance:
The combined effect of silver nanoparticles and laser disinfection against E. faecalis holds a promising treatment modality for eradicating resistant pathogens and biofilms embedded deep inside the dentinal tubules that are not amenable to conventional disinfection protocols in root canals.
