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GLC determination for ftorafur in biological fluids.

E B Hills, V C Godefroi, I A O'Leary

    Journal of Pharmaceutical Sciences
    |October 1, 1977
    PubMed
    Summary

    A new GLC method accurately measures free ftorafur in patient samples. This drug analysis is sensitive, specific, and does not affect later fluorouracil testing.

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    Area of Science:

    • Pharmacokinetics
    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Ftorafur is a prodrug converted to 5-fluorouracil, used in cancer therapy.
    • Monitoring drug disposition is crucial for effective patient treatment.
    • Accurate quantification of free ftorafur is needed to understand its pharmacokinetics.

    Purpose of the Study:

    • To develop and validate a gas-liquid chromatography (GLC) method for quantifying free ftorafur.
    • To enable the tracking of drug disposition in biological fluids from patients.
    • To ensure the method is sensitive, specific, and compatible with subsequent analyses.

    Main Methods:

    • Free ftorafur was extracted from aqueous biological samples using chloroform.
    • The extracted drug was derivatized via methylation.

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  • Chromatography was performed on a 1% HI-EFF 8BP column with flame-ionization detection.

    • Main Results:

    • The GLC method demonstrated high sensitivity, with a limit of 0.25 microgram/ml in plasma.
    • The analysis proved specific for the intact ftorafur molecule.
    • The method showed no interference with subsequent fluorouracil analysis in the same samples.

    Conclusions:

    • A reliable GLC method for free ftorafur analysis in biological fluids has been established.
    • This method facilitates the study of ftorafur drug disposition in patients.
    • The validated assay is suitable for clinical pharmacokinetic studies.