The correction for extraneous background in quantitative X-ray microanalysis of biological thin sections: some...

Area of Science:

• Materials Science
• Microscopy
• Analytical Chemistry

Background:

• Quantitative analysis of biological tissue sections relies on accurate background continuum correction.
• Existing theoretical models for continuum correction face practical challenges in application.
• Electron microscopy and X-ray analysis are crucial for elemental quantification in biological samples.

Purpose of the Study:

• To identify sources of inaccuracy in quantitative electron microscopy procedures.
• To investigate the impact of geometrical factors on continuum correction in biological tissue analysis.
• To propose improvements for accurate X-ray analysis of thin sections.

Main Methods:

• Utilized a model system of homogeneous plastic sections on copper mesh grids.
• Employed an unmodified electron microscope under standard analytical conditions.
• Analyzed geometrical factors influencing X-ray analysis and continuum correction.

Main Results:

• Geometrical factors, particularly analysis position relative to grid bars, are critical for accuracy.
• Support film continuum correction must match the analysis location relative to grid bars.
• Specimen holder absorption and differential mass loss also introduce significant errors.

Conclusions:

• Precise positioning during X-ray analysis is essential for accurate continuum correction.
• Slot grids may offer advantages over mesh grids in reducing geometrical artifacts.
• Addressing geometrical factors, mass loss, and specimen current variations is key to reliable quantitative analysis.