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Updated: Nov 14, 2025

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Published on: October 28, 2011
Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development
Matthias Jung1, Jovita Schiller2, Carla Hartmann2
1Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Psychiatry, Psychotherapy, and Psychosomatic Medicine, Halle/Saale, Germany. matthias.jung@uk-halle.de.
Abstract:
We describe the protocol for the efficient in vitro differentiation of human neural stem cells (NSCs) from human-induced pluripotent stem cells (iPS cells). NSCs differentiate via neural epithelial progenitors enabling the analysis of early neuronal development. They represent neural progenitor cells, which are capable of differentiating into neurons and glia.
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