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Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
Published on: March 12, 2020
Genome Mining Discovery of a C4-Alkylated Dihydroisocoumarin Pathway in Fungi
1College of Pharmaceutical Sciences, Medical Research Institute, Southwest University, Chongqing 400715, China.
Abstract:
A fungal C4-alkylated dihydroisocoumarin pathway was discovered and elucidated. This pathway includes the following (1) a nonreducing polyketide synthase and a P450 collaboratively synthesize hydroxylated C3-methylated isocoumarin 3; (2) a methyltransferase methylates 3 to 8; and (3) importantly, an esterase specifically catalyzes a ring reconstruction process of 8 to C4-alkylated dihydroisocoumarin 10. Our discovery fills the gap in the enzymatic transformation process of natural C4-alkylated isocoumarin derivates.
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