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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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In-situ Hybridization02:31

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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STRide probes: Single-labeled short tandem repeat identification probes.

Olivier Tytgat1, Maarten Fauvart2, Tim Stakenborg2

  • 1Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460, Gent, 9000, Belgium; Imec, Kapeldreef 75, Leuven, 3001, Belgium.

Biosensors & Bioelectronics
|March 10, 2021
PubMed
Summary

Forensic DNA profiling is advancing with a new hybridization method using Short Tandem Repeat Identification (STRide) probes. This technique enables rapid, on-site DNA fingerprinting, overcoming limitations of current laboratory-based methods.

Keywords:
ForensicsLab-on-a-chipShort tandem repeat genotyping

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Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Forensic DNA profiling demand is rising, requiring specialized labs and bulky equipment.
  • Current methods involve Short Tandem Repeat (STR) amplification and capillary electrophoresis.
  • Decentralized, on-site DNA analysis is hindered by equipment size and complexity.

Purpose of the Study:

  • To develop a novel, portable method for forensic DNA profiling.
  • To address the limitations of current laboratory-based DNA analysis workflows.
  • To enable faster, on-site DNA fingerprinting.

Main Methods:

  • Developed a hybridization-based Short Tandem Repeat Identification (STRide) probe method.
  • Utilized guanine quenching properties and melting curve analysis after asymmetric PCR.
  • Designed and validated STRide probes for five CODIS core loci.

Main Results:

  • Demonstrated functionality with synthetic DNA for the D16S539 locus.
  • Successfully genotyped 13 human DNA samples with high sensitivity (as low as 31 pg DNA).
  • Achieved successful genotyping for CODIS core loci: D16S539, TH01, TPOX, FGA, and D7S820.

Conclusions:

  • STRide probes offer a sensitive and specific method for forensic DNA genotyping.
  • The method is suitable for microarray implementation, paving the way for portable devices.
  • This represents a significant advancement towards rapid, on-site forensic DNA fingerprinting.