Rapid and label free detection of aflatoxin B₁ in alcoholic beverages with a microfluid fiber device

A rapid and label free aflatoxin B₁ (AFB₁) microfluid sensor was proposed and tested. The device was fabricated with hollow-core photonics crystal fiber infiltrated with the AFB₁ solution. The autofluorescence emitting from the AFB₁ molecules was detected. The sensor length was optimized. The AFB₁ concentration was tested with a 4 cm long sensor. The best limit of detection was achieved as low as 1.34 ng/ml, which meets the test requirement of the national standards for AFB₁ in food. The effectiveness of this sensor being applied in beer solution was also verified to be a little more sensitive than in aqueous solution. Compared with traditional AFB₁ detection methods, the proposed single-ended device perfectly satisfies the demand of process control in alcoholic beverages manufacture.