Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fourier-basis active structured illumination sensing lidar imaging using crossed acousto-optic devices.

Applied optics·2026
Same author

Time-shifted pseudo-noise multibeam lidar array using acousto-optic deflectors.

Applied optics·2025
Same author

Improving Management and Compliance of Radiation Therapy Linear Accelerator Quality Assurance Program With Automated Tracking Tools.

Advances in radiation oncology·2024
Same author

Terahertz imaging using optically controlled Fourier-basis structured illumination.

Applied optics·2022
Same author

Monopulse ladar: super-resolved 3D localization with Si-photonic serpentine optical phased arrays.

Applied optics·2022
Same author

Vernier optical phased array lidar transceivers.

Optics express·2022
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Nov 14, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.7K

Afocal catadioptric optical assembly for Fourier-sampling computational microscopy.

Keith Nowicki, Kelvin H Wagner, Robert Cormack

    Applied Optics
    |March 10, 2021
    PubMed
    Summary
    This summary is machine-generated.

    A novel optical assembly projects coherent beams for computational microscopy, enabling variable imaging distances. This fast, wide-angle system meets unique demands unmet by traditional designs.

    More Related Videos

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.8K
    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
    07:19

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

    Published on: June 28, 2017

    10.6K

    Related Experiment Videos

    Last Updated: Nov 14, 2025

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

    Published on: August 4, 2018

    8.7K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.8K
    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
    07:19

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

    Published on: June 28, 2017

    10.6K

    Area of Science:

    • Optical Engineering
    • Microscopy

    Background:

    • Traditional imaging designs struggle with the unique optical requirements of Fourier-sampling computational microscopy.
    • Demands include projection of coherent collimated beams with specific input and output characteristics.

    Purpose of the Study:

    • To describe a fast, wide-angle, afocal, catadioptric optical assembly for computational microscopy.
    • To address the unmet optical requirements of Fourier-sampling microscopy.

    Main Methods:

    • Design and simulation of a catadioptric optical assembly.
    • Derivation of equations for focal surfaces relevant to system alignment.
    • Analysis of optical performance for beam interferometry.

    Main Results:

    • The system accepts diverging collimated beams and produces converging collimated beams overlapping at a target.
    • Simulations confirm optical performance for axially symmetric and asymmetric beam interferometry.
    • A method to adjust microscope imaging distance by up to one meter was demonstrated.

    Conclusions:

    • The developed optical assembly successfully meets the unorthodox requirements for Fourier-sampling computational microscopy.
    • The system offers flexibility in imaging distance adjustment through minor optical element shifts.
    • This design advances capabilities in specialized microscopy applications.