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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: Nov 14, 2025

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Inline holographic microscopy through fiber imaging bundles.

Michael R Hughes

|March 10, 2021

Summary

This summary is machine-generated.

This study introduces a simple inline holographic microscopy method for fiber imaging bundles. This technique enables volumetric imaging with high resolution, overcoming challenges in miniaturized microscopy without complex calibration.

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Area of Science:

Optics and Photonics
Biomedical Imaging
Microscopy

Background:

Fiber imaging bundles are crucial for endoscopic and miniaturized microscopy, especially for fluorescence imaging.
Holographic microscopy via fiber bundles is difficult due to complexity and calibration needs of phase conjugation methods.

Purpose of the Study:

To develop a simple inline holographic microscopy technique for imaging bundles.
To enable volumetric imaging through fiber bundles without extensive calibration.

Main Methods:

Utilized a partially coherent illumination source from a multimode fiber for inline holographic microscopy.
Imaged samples in transmission, capturing intensity holograms with the fiber bundle.
Employed numerical refocusing for volumetric reconstruction.

Main Results:

Achieved a resolution of approximately 6 µm.
Demonstrated volumetric imaging over a depth range of 1 mm.
The method proved insensitive to fiber bending due to lack of complex calibration.

Conclusions:

Simple inline holographic microscopy is feasible through imaging fiber bundles.
This technique offers a robust and calibration-free approach for miniaturized volumetric imaging.