Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-speed hyperspectral single-pixel microscopy via line-scan detection with data fusion-based enhanced resolution.

Communications engineering·2026
Same author

Design, construction and validation of an open-source gold nanoparticle concentration tracking device.

HardwareX·2026
Same author

Hadamard based single-pixel microscopy using sensor-less adaptive optics supported by multi-actuator adaptive lens.

Nature communications·2025
Same author

Quantitative phase imaging in Hadamard-based active single-pixel microscopy by the motionless transport of intensity equation.

Optics letters·2025
Same author

Simulation of digital lensless holographic microscopy holograms: a physics-image processing approach.

Optics express·2025
Same author

Optical characterization of native aerosols from e-cigarettes in localized volumes.

Biomedical optics express·2024

Related Experiment Video

Updated: Nov 14, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.5K

Open-source, cost-effective, portable, 3D-printed digital lensless holographic microscope.

Heberley Tobon-Maya, Samuel Zapata-Valencia, Erick Zora-Guzmán

    Applied Optics
    |March 10, 2021
    PubMed
    Summary

    This study presents the most affordable digital lensless holographic microscope (DLHM), constructed using 3D printing and readily available materials for under $53. The cost-effective DLHM platform demonstrates versatile imaging capabilities for various samples.

    More Related Videos

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.8K
    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
    05:45

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

    Published on: March 31, 2022

    2.9K

    Related Experiment Videos

    Last Updated: Nov 14, 2025

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.5K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.8K
    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
    05:45

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

    Published on: March 31, 2022

    2.9K

    Area of Science:

    • Optics and Photonics
    • Microscopy Engineering
    • Biomedical Imaging

    Background:

    • Traditional microscopy techniques can be expensive and complex.
    • Digital Lensless Holographic Microscopy (DLHM) offers a promising alternative for cost-effective imaging.
    • There is a need for highly accessible and versatile microscopic tools in research and education.

    Purpose of the Study:

    • To design, construct, and test the most cost-effective Digital Lensless Holographic Microscope (DLHM) to date.
    • To demonstrate the feasibility of using 3D-printed components and off-the-shelf materials for microscope construction.
    • To validate the performance of the developed DLHM for imaging diverse biological and non-biological samples.

    Main Methods:

    • Utilized 3D printing and off-the-shelf components to build a DLHM setup.
    • Employed open-source software for processing recorded in-line holograms.
    • Configured the DLHM for variable fields of view, spatial resolution, and 2D sample scanning.

    Main Results:

    • Successfully constructed a functional DLHM microscope for a total cost of $52.82.
    • Demonstrated the capability to image resolution test targets, a Drosophila melanogaster fly head section, red blood cells, and cheek cells.
    • The DLHM setup exhibited flexibility in adjusting imaging parameters.

    Conclusions:

    • The developed DLHM represents a significant advancement in low-cost, high-performance microscopy.
    • This affordable platform can be adapted for various imaging applications, democratizing access to advanced microscopy.
    • The 3D-printed DLHM is a viable tool for educational and research purposes, particularly in resource-limited settings.