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Updated: Nov 14, 2025
A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
A coumarin-based "off-on" fluorescent probe for highly selective detection of hydrogen sulfide and imaging in living
Luying Ou1, Rui Guo, Weiying Lin
1Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China.
Abstract:
Hydrogen sulfide (H2S), as a significant signaling molecule, is associated with diverse physiological and pathological processes. However, it's still a challenge to explore outstanding tools for detecting endogenous H2S in vivo. Thus, a simple "off-on" H2S fluorescent probe CMHS has been reasonably designed, and it is based on coumarin as the fluorophore group. The probe CMHS displayed a crucial turn-on fluorescence enhancement (180-fold), rapid reaction time, high selectivity, and a low limit of detection (2.31 × 10-7 M). Additionally, probe CMHS could be applied to visualize exogenous and endogenous H2S successfully in HeLa cells with low cytotoxicity and good permeability.
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