Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

2.8K
Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
2.8K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.2K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.2K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.8K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.8K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

25.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
25.0K
Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

3.3K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
3.3K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.2K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sensors without Borders.

ACS sensors·2026
Same author

Oppositely Charged Single Enzyme Nanogels Form Versatile Coacervates for Efficient Enzyme Cascade Catalysis.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Engineering a Transmembrane Receptor for Coacervate-Based Artificial Cells.

Journal of the American Chemical Society·2026
Same author

Light-tunable DNA interactions enable spatiotemporal assembly and relaxation-driven crystallization of colloids.

Soft matter·2026
Same author

Thermostable Bioluminescent Intercalating Dyes for Real-Time, Integrated Nucleic Acid Amplification and Detection.

Angewandte Chemie (International ed. in English)·2026
Same author

Homogeneous Antibody-DNA Conjugates Using Unmodified Oligonucleotides and Photo-Cross-Linkable Protein G-HUH Endonuclease Fusion Proteins.

Bioconjugate chemistry·2026

Related Experiment Video

Updated: Nov 14, 2025

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.1K

Pathway-Dependent Co-Assembly of Elastin-Like Polypeptides.

Jan Pille1, Antonio Aloi1, Duc H T Le1

  • 1Department of Biomedical Engineering & Department of Chemical Engineering and Chemistry, Institute for Complex Molecular Systems Eindhoven University of Technology, PO Box 513, Eindhoven, 5600 MB, the Netherlands.

Small (Weinheim an Der Bergstrasse, Germany)
|March 10, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method for creating tunable polymer particles using stimulus-responsive polymers. This pathway-dependent co-assembly allows for controlled particle formation and efficient encapsulation of biomolecules for cellular delivery.

Keywords:
elastin-like polypeptidespathway-dependent assemblypolymersself-assembly

More Related Videos

Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

9.1K
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

22.2K

Related Experiment Videos

Last Updated: Nov 14, 2025

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.1K
Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

9.1K
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

22.2K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Biotechnology

Background:

  • Natural systems exhibit temperature-induced assembly of biomolecules into distinct states based on heating trajectory.
  • Achieving similar pathway-dependent assembly in synthetic polymer mixtures has been challenging.

Purpose of the Study:

  • To demonstrate a novel pathway-dependent assembly in synthetic polymer mixtures.
  • To create tunable, co-assembled particles for efficient biomolecular encapsulation and delivery.

Main Methods:

  • Utilizing a mixture of mono- and diblock copolymers based on elastin-like polypeptides.
  • Controlling particle formation through a critical heating rate during thermal processing.
  • Encapsulating fluorescent proteins and bioluminescent enzymes within the co-assembled particles.

Main Results:

  • Achieved monodisperse, stable co-assembled particles with tunable hydrodynamic radii (20-120 nm) at a critical heating rate.
  • Demonstrated that below the critical rate, constituents form separate assemblies.
  • Showcased high encapsulation efficiency for fluorescent proteins and bioluminescent enzymes.
  • Confirmed unperturbed function of encapsulated cargo after cellular delivery.

Conclusions:

  • Pathway-dependent co-assembly of elastin-like polypeptides offers fundamental insights into materials science, enabling multiple distinct assemblies from the same precursors.
  • This method provides a versatile platform for constructing efficient vehicles for cellular delivery of biomolecular cargo.