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Related Experiment Video

Updated: Nov 14, 2025

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis.

Daishi Yamakawa¹, Daisuke Katoh², Kousuke Kasahara¹

¹Department of Physiology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan.

|March 10, 2021

Summary

Primary cilia regulate fat cell development by controlling lipid raft dynamics. Disrupting cilia formation impairs adipogenesis, offering new insights into metabolic regulation.

Keywords:

Akt adipogenesis caveolae ciliary base insulin signaling lipid rafts obesity primary cilia trichoplein

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Published on: February 3, 2023

Lipid Exchange Assay in Living Cells

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Published on: March 21, 2025

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Last Updated: Nov 14, 2025

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

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Published on: March 17, 2023

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Lipid Exchange Assay in Living Cells

Lipid Exchange Assay in Living Cells

Published on: March 21, 2025

Area of Science:

Cell Biology
Metabolic Signaling
Developmental Biology

Background:

Primary cilia are crucial signaling hubs involved in development.
Primary cilium dysfunction is linked to altered adipogenesis, but mechanisms remain elusive.

Purpose of the Study:

To elucidate the role of primary cilia in adipogenesis.
To investigate the molecular mechanisms by which cilia influence fat cell differentiation.

Main Methods:

Utilized mesenchymal progenitor cells (C3H10T1/2) with trichoplein depletion.
Generated trichoplein knockout mice.
Analyzed cilia length, lipid raft dynamics, insulin signaling, and adipogenesis.
Assessed body fat and adipocyte size in mice on a high-fat diet.

Main Results:

Trichoplein-depleted cells and knockout mice exhibited elongated primary cilia.
Elongated cilia inhibited insulin receptor accumulation in lipid rafts, blocking insulin-Akt signaling.
Mice with impaired cilia showed reduced body fat and smaller adipocytes, even on a high-fat diet.

Conclusions:

Primary cilia regulate adipogenic signal transduction through control of lipid raft dynamics.
Cilia length and associated lipid raft assembly are critical for proper adipogenesis.
Targeting primary cilia may offer novel strategies for metabolic disease treatment.