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Mitogen-Inducible Gene 6 Inhibits Angiogenesis by Binding to SHC1 and Suppressing Its Phosphorylation

Lixian Liu1, Liying Xing1, Rongyuan Chen1

  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Frontiers in Cell and Developmental Biology
|March 11, 2021
PubMed

Insights

Mitogen-inducible gene 6 (MIG6) inhibits blood vessel formation. This study reveals MIG6

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Vascular Biology

Background:

  • Mitogen-inducible gene 6 (MIG6) is an adaptor protein found in vascular endothelial cells.
  • The role of MIG6 in angiogenesis, the formation of new blood vessels, is currently unknown.

Purpose of the Study:

  • To investigate the function of MIG6 in retinal development and neovascularization.
  • To elucidate the molecular and cellular mechanisms underlying MIG6's effect on angiogenesis.

Main Methods:

  • Utilized in vitro and in vivo model systems to study angiogenesis.
  • Performed loss-of-function assays (genetic deletion, siRNA knockdown) and overexpression studies.
  • Investigated the interaction between MIG6 and SHC1 in endothelial cells (ECs).

Main Results:

  • Loss of MIG6 function led to increased angiogenesis in vitro and in vivo.
  • MIG6 overexpression suppressed pathological angiogenesis.
  • MIG6 directly inhibits vascular endothelial cells by binding to and inhibiting SHC1 phosphorylation.

Conclusions:

  • MIG6 acts as a potent endogenous inhibitor of angiogenesis.
  • MIG6's anti-angiogenic effect is mediated through the SHC1 pathway.
  • MIG6 holds potential therapeutic value for anti-angiogenic therapies.

Keywords:
MIG6SHC1angiogenesisendothelial cellocular neovascularization

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