Related Experiment Video
Updated: Nov 14, 2025
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Published on: June 5, 2020
FRC-QE: a robust and comparable 3D microscopy image quality metric for cleared organoids
Friedrich Preusser1, Natália Dos Santos2, Jörg Contzen3
1Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin 10115, Germany.
Summary:
Here, we propose Fourier ring correlation-based quality estimation (FRC-QE) as a new metric for automated image quality estimation in 3D fluorescence microscopy acquisitions of cleared organoids that yields comparable measurements across experimental replicates, clearing protocols and works for different microscopy modalities.
Availability And Implementation:
FRC-QE is written in ImgLib2/Java and provided as an easy-to-use and macro-scriptable plugin for Fiji. Code, documentation, sample images and further information can be found under https://github.com/PreibischLab/FRC-QE.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
