Ratiometric fluorescence for sensitive detection of phosphate species based on mixed lanthanide metal organic...

Phosphate (PO₄^3-) plays a major role in aquatic ecosystems and biosystems. Developing a highly sensitive and selective ratiometric fluorescence probe for detection of PO₄^3- is of great significance to the ecological environment and human health. In this work, a novel dual lanthanide metal organic framework was synthesized via hydrothermal reaction based on Tb³⁺ and Ce³⁺ as the center metal ions and terephthalic acid as the organic ligand (designated as Tb-Ce-MOFs). The fluorescence of Tb-Ce-MOFs shows emission at 375 nm. In the presence of PO₄^3-, with increased concentration of PO₄^3-, the fluorescence intensity of Tb-Ce-MOFs at 500 nm and 550 nm increased, while the intensity at 375 nm was reduced. Hence, ratiometric fluorescence detecting of PO₄^3- can be achieved by measuring the ratio of fluorescence at 550 nm (FL₅₅₀) to 375 nm (FL₃₇₅) in the fluorescent spectra of the Tb-Ce-MOFs. In this sensing approach, the Tb-Ce-MOFs probe exhibits highly sensitive and selective for detection of PO₄^3-. The limit of detection is calculated to be 28 nM and the detection range is 0.1 to 10 μM. In addition, the Tb-Ce-MOFs were used in the detection of PO₄^3- in real samples. We design and synthesize a mixed lanthanide metal organic framework fluorescence probe (Tb-Ce-MOFs) for ratiometric fluorescence for the detection of PO₄^3- based on Tb³⁺ and Ce³⁺ as the center metal ions and terephthalic acid as the organic ligand.