Comparative assessment of cell/substratum static adhesion using an in vitro organ culture method and computerized...

The trypsin sensitivity of chick embryo cellular layers cultivated by an in vitro organ culture method toward different biomaterials has been analysed. Monolayer cells grown on to tested samples were enzymatically dissociated in 5 min - 1 h. Cumulative cell numbers, expressed as the percentage of the totally detached cell number, were plotted versus time, thus permitting the calculation of the function. The mathematical treatment by a computerized system of this function represents the trypsin sensitivity. This value modulated by the migration cell number is the static adhesion modulated index (SAMI). The trypsin sensitivity expressed according to this index allowed the establishment of an abacus wherein several zones, A, B, C and D, define cell adhesion behaviour on different biomaterials. Scanning electron microscopy analysis performed on dissociation steps showed the selective activity of trypsin on cells toward different substrata, revealing the role of an extracellular matrix and cytoskeleton in the adhesion behaviour.