Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

16.4K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
16.4K
Cross-reactivity00:42

Cross-reactivity

32.0K
Overview
32.0K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.8K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.8K
Antibody Structure01:10

Antibody Structure

62.7K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
62.7K
Immunoprecipitation01:20

Immunoprecipitation

6.3K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
6.3K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

1.1K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The mutational landscape of STING-induced immunity.

Nature·2026
Same author

Prediction of pre- and postfusion conformations of class I fusion proteins with AlphaFold2.

PloS one·2026
Same author

Blocking CD30 on CD19 CAR T cells augments their functional capacities against B-cell leukemia/lymphoma.

Frontiers in immunology·2026
Same author

Capsid-engineered AAV vector overcomes a key intracellular barrier and efficiently transduces spiral ganglion neurons in adult mice.

Molecular therapy. Advances·2026
Same author

Sequence to structure insights into Lassa virus population-level biophysical properties and glycoprotein structure catalogue.

Npj viruses·2026
Same author

VUStruct: A compute pipeline for high throughput and personalized structural biology.

PLoS computational biology·2026

Related Experiment Video

Updated: Nov 14, 2025

Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

12.6K

Modeling Immunity with Rosetta: Methods for Antibody and Antigen Design.

Clara T Schoeder1,2, Samuel Schmitz1,2, Jared Adolf-Bryfogle3,4

  • 1Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37212, United States.

Biochemistry
|March 11, 2021
PubMed
Summary

Computational methods in Rosetta enable advanced antibody and antigen design. This guide provides tutorials for structure prediction, docking, and design, including glycan addition, for novice users.

More Related Videos

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

12.7K
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

13.1K

Related Experiment Videos

Last Updated: Nov 14, 2025

Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

12.6K
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

12.7K
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

13.1K

Area of Science:

  • Computational Biology
  • Structural Biology
  • Immunology

Background:

  • Advances in experimental structure determination and computational methods have propelled structure-based antibody and antigen design.
  • The Rosetta software suite offers robust tools for biomolecular modeling, including prediction, docking, and design applications.

Purpose of the Study:

  • To provide an overview of current Rosetta protocols for antibody and antigen modeling.
  • To offer detailed tutorials for novice users to apply Rosetta in their own antibody and antigen modeling projects.

Main Methods:

  • Utilized Rosetta software suite for biomolecular modeling.
  • Developed detailed tutorials covering antibody structure prediction, docking, and design.
  • Included antigen design strategies and glycan addition protocols within Rosetta.

Main Results:

  • Presented comprehensive protocols for antibody and antigen modeling using Rosetta.
  • Demonstrated practical applications through detailed workshop-developed tutorials.
  • Enabled novice users to perform antibody and antigen structure prediction, docking, and design.

Conclusions:

  • Rosetta provides a versatile platform for advanced antibody and antigen design.
  • The provided tutorials facilitate the adoption of Rosetta for structure-based modeling by a wider user base.
  • This work empowers researchers to explore antibody-antigen interactions and design novel therapeutics.