Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative microscopy of enzyme reactions in tissue sections.

F P Altman

    Microscopica Acta
    |July 1, 1977
    PubMed
    Summary

    Microdensitometry allows quantitative measurement of enzyme reactions in specific tissue regions. This technique links cellular enzyme activity directly to histological features for detailed analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Enzyme histochemical changes in murine osteoarthritis.

    Histochemistry·1982
    Same author

    A metabolic dysfunction in early murine osteoarthritis.

    Annals of the rheumatic diseases·1981
    Same author

    Some aspects of the use of tetrazolium salts in quantitative histochemistry.

    Acta histochemica. Supplementband·1981
    Same author

    Oxidative enzyme metabolism in rabbit intrasynovial flexor tendons. I. Changes in enzyme activity of the tenocytes with age.

    The Journal of surgical research·1980
    Same author

    Oxidative enzyme metabolism in rabbit intrasynovial flexor tendons. II. Studies of nutritional pathways.

    The Journal of surgical research·1980
    Same author

    Oxidative enzyme activity in rabbit intrasynovial flexor tendons. III. Changes in enzyme activity of hypovascular tendons after physical activity.

    The Journal of surgical research·1980

    Area of Science:

    • Histochemistry
    • Biomedical Imaging
    • Cellular Biology

    Background:

    • Quantitative analysis of enzyme activity in tissues is crucial for understanding biological processes.
    • Traditional methods may lack the spatial resolution to correlate enzyme activity with specific cellular structures.

    Purpose of the Study:

    • To introduce and illustrate the application of microdensitometry for quantitative analysis of enzyme reaction products in tissue sections.
    • To demonstrate the ability to obtain quantitative data from discrete cellular regions and relate it to histology.

    Main Methods:

    • Microdensitometry was employed to measure reaction products within selected regions of tissue sections.
    • The technique allows for the precise selection of single cells or groups of cells for analysis.

    Main Results:

    • Microdensitometry provides quantitative data on enzyme reaction products in defined tissue areas.
    • The method successfully relates enzyme activity levels to specific histological features.

    Conclusions:

    • Microdensitometry is a valuable technique for quantitative histochemistry.
    • It enables precise correlation of enzyme activity with cellular morphology, enhancing biological insights.

    Related Experiment Videos