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Soybean-derived gma-miR159a alleviates colon tumorigenesis by suppressing TCF7/MYC in mice

Juncheng Liu1, Fang Wang1, Haizhao Song1

  • 1College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China.

The Journal of Nutritional Biochemistry
|March 11, 2021
PubMed

Insights

Dietary intake of soybean microRNA (gma-miR159a) effectively prevents colon cancer and colitis. This plant-derived miRNA targets the TCF7 gene, suppressing oncogene MYC and reducing tumor growth in mice.

Area of Science:

  • Molecular Biology
  • Oncology
  • Nutrition Science

Background:

  • Plant-derived microRNAs (miRNAs) can regulate mammalian gene expression post-dietary intake.
  • Soybean-derived gma-miR159a was previously shown to inhibit colon cancer cell proliferation.

Purpose of the Study:

  • To investigate the anti-colon cancer effects of dietary gma-miR159a in a mouse model.
  • To elucidate the molecular mechanisms underlying gma-miR159a's anti-cancer activity.

Main Methods:

  • Azoxymethane (AOM)/dextran sodium sulfate (DSS) induced colon cancer mouse model.
  • In vitro studies on colon cancer cell lines to assess gma-miR159a's effect on MYC and TCF7.
  • In vivo gavage administration of gma-miR159a and soybean RNA in AOM/DSS mice, with and without anti-miR159a.

Main Results:

  • gma-miR159a demonstrated stability in cooked soybean.
  • In vitro, gma-miR159a suppressed MYC expression by targeting TCF7, inhibiting colon cancer cell growth.
  • In vivo, gma-miR159a and soybean RNA significantly reduced tumor growth and attenuated inflammation in AOM/DSS mice.
  • The anti-cancer effect was abolished by the presence of anti-miR159a.

Conclusions:

  • Dietary gma-miR159a effectively prevents colon cancer and colitis in mice.
  • Soybean-derived gma-miR159a offers a novel dietary strategy for colon cancer prevention.
Keywords:
Colon cancerMicroRNAsSoybeanTCF7gma-miR159a

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