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Related Experiment Video

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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Refining a correlative light electron microscopy workflow using luminescent metal complexes.

Jonathan R Shewring1, Lorna Hodgson2, Helen L Bryant3

  • 1Department of Chemistry, University of Sheffield, Sheffield, United Kingdom.

Methods in Cell Biology
|March 12, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel iridium-based metal complex for Correlative Light Electron Microscopy (CLEM). This new probe overcomes limitations of existing tools, enabling seamless imaging across multiple scales in life science research.

Keywords:
CLEMFinder dishesImaging dishesLuminescent metal complexRetracing

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Area of Science:

  • Life Science Research
  • Microscopy Technologies
  • Nanotechnology

Background:

  • Correlative Light Electron Microscopy (CLEM) is crucial for life science research, bridging macro and nano-scale imaging.
  • Current molecular probes for CLEM face limitations: organic probes lack electron scattering, while metal compounds are non-luminescent.
  • Existing probes like gold nanoparticles and quantum dots have significant drawbacks for CLEM applications.

Purpose of the Study:

  • To address the limitations of current molecular probes for CLEM.
  • To develop a novel luminescent probe suitable for both light and electron microscopy.
  • To establish a robust CLEM workflow utilizing advanced metal complexes.

Main Methods:

  • Development of an iridium-based luminescent metal complex (Ir complex 1).
  • Characterization of the probe's emissive and electron-scattering properties.
  • Establishment of a CLEM workflow integrating the novel probe.

Main Results:

  • The iridium-based complex exhibits both luminescence and good electron scattering properties.
  • The developed probe facilitates imaging across a wide range of scales (microns to nanometers).
  • The new CLEM workflow demonstrates effective application of the metal complex probe.

Conclusions:

  • Iridium-based luminescent metal complexes offer a promising solution for advanced CLEM applications.
  • The developed probe and workflow overcome key challenges in multi-scale imaging.
  • This advancement has the potential to significantly increase the application of CLEM in life sciences.