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Complete Genome Sequence of Stenotrophomonas maltophilia Siphophage Salva

Brandi Jefferson1, Guichun Yao2, James Clark3

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|March 12, 2021
PubMed

Insights

Researchers sequenced the genome of phage Salva, a novel bacteriophage targeting Stenotrophomonas maltophilia. This discovery offers new insights into S. maltophilia phages, potentially aiding in combating infections.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriophage Research

Background:

  • Stenotrophomonas maltophilia is a Gram-negative bacterium responsible for severe, difficult-to-treat infections like pneumonia and bacteremia.
  • Novel therapeutic strategies are needed to combat infections caused by antibiotic-resistant pathogens like S. maltophilia.

Purpose of the Study:

  • To present the complete genome sequence of a novel bacteriophage, Salva, targeting Stenotrophomonas maltophilia.
  • To characterize the genetic makeup of phage Salva and assess its novelty compared to existing phage databases.

Main Methods:

  • Genome sequencing of the novel bacteriophage Salva.
  • Bioinformatic analysis to identify putative protein-coding genes.
  • Comparative genomic analysis against existing phage sequences in GenBank.

Main Results:

  • The genome of phage Salva was sequenced and found to be 60,789 base pairs in length.
  • The genome contains 102 putative protein-coding genes.
  • Phage Salva is not closely related to any previously characterized S. maltophilia phages in GenBank, indicating its novelty.

Conclusions:

  • The characterization of phage Salva provides a new genomic resource for S. maltophilia research.
  • This novel phage represents a potential candidate for phage therapy against S. maltophilia infections.
  • Further studies are warranted to explore the lytic activity and therapeutic potential of phage Salva.

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