Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

c-Met-targeted NIR-II imaging for precision management of oral squamous cell carcinoma and premalignant lesions.

Theranostics·2026
Same author

Concurrent Evaluation of Liver and Kidney Function by NIR-II <i>In Vivo</i> Imaging to Assess the Efficacy of Flavonoids in Preventing Their Injuries.

Chemical & biomedical imaging·2026
Same author

Development of Glycosylated and Dimeric FAP-Targeted Radiotracers for Preclinical Evaluation in FAP-Positive Glioblastoma Models.

Journal of medicinal chemistry·2026
Same author

Pathology-Responsive Nanoprobes for NIR-II Imaging of Acute Kidney Injury.

Analytical chemistry·2026
Same author

Harmonizing High Phosphorescence Efficiency and Stretchability in Flexible Afterglow Materials Through Microphase Engineering.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Novel Carboline-Based Ferroptosis Inhibitor Ameliorates Acute Liver Injury by Remodeling Phosphatidylcholine.

Journal of medicinal chemistry·2026

Related Experiment Video

Updated: Nov 13, 2025

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.6K

Smart Self-Assembly Amphiphilic Cyclopeptide-Dye for Near-Infrared Window-II Imaging.

Hao Chen1, Kangquan Shou2, Si Chen2

  • 1Center for Molecular Imaging Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Advanced Materials (Deerfield Beach, Fla.)
|March 12, 2021
PubMed
Summary

A novel pH-sensitive nanoprobe, SIMM1000, enables enhanced second near-infrared window (NIR-II) imaging of diseases. This smart molecule self-assembles in acidic tumor environments, improving imaging contrast and retention for precision medicine.

Keywords:
NIR-II fluorescence imagingfluorescence-imaging-guided tumor surgerypH-responsive materialsself-assemblytumor imaging

More Related Videos

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.1K
Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies
08:54

Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

Published on: February 17, 2023

1.3K

Related Experiment Videos

Last Updated: Nov 13, 2025

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.6K
A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.1K
Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies
08:54

Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

Published on: February 17, 2023

1.3K

Area of Science:

  • Chemistry
  • Precision Medicine
  • Nanomaterials Science

Background:

  • Development of novel nanomaterials is crucial for disease theranostics.
  • Fluorescent probes in the second near-infrared window (NIR-II) offer high sensitivity, resolution, and deep tissue penetration for biomedical imaging.

Purpose of the Study:

  • To develop a smart, pH-sensitive self-assembling nanoprobe for enhanced NIR-II imaging of diseases in vivo.
  • To investigate the aggregation behavior and fluorescence properties of the nanoprobe in response to tumor microenvironment pH changes.

Main Methods:

  • Synthesis and characterization of a cyclopeptide-dye conjugate (SIMM1000).
  • Evaluation of SIMM1000 self-assembly and fluorescence enhancement in response to pH variations.
  • In vivo NIR-II imaging of blood vessels, bone, and tumors in mice.

Main Results:

  • SIMM1000 self-assembles from 80 nm to over 500 nm in acidic conditions (pH 7.0 to 6.8), with a 20-30 fold fluorescence enhancement.
  • Achieved micrometer-scale resolution in blood vessel imaging and high-contrast imaging of bone and tumors.
  • Demonstrated significant tumor accumulation and prolonged retention (>19 days) with safe excretion from normal tissues.

Conclusions:

  • The pH-sensitive self-assembling nanoprobe SIMM1000 offers a promising strategy for advanced NIR-II molecular imaging.
  • This approach can enhance tumor visualization, retention, and theranostic applications.
  • The smart self-assembling small molecule design paradigm holds potential for future nanomaterial development in imaging and drug delivery.