Antibacterial Activity and Mechanism of Coenzyme Q₀ Against Escherichia coli

Coenzyme Q₀ (CoQ₀) is a natural compound found in Antrodia cinnamomea, which has a variety of biological activities. Here, the antibacterial activity and possible antibacterial mechanism of CoQ₀ against Escherichia coli were investigated. The antibacterial effect was evaluated by determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, and by assessing bacterial survival and the effect on the growth of E. coli after CoQ₀ treatment in Luria-Bertani (LB) broth. To reveal the antibacterial mechanism of CoQ₀, changes in intracellular adenosine triphosphate (ATP) concentration, membrane potential, and bacterial protein content, as well as effects on cell morphology and membrane integrity, were investigated. Both the MICs and MBCs of CoQ₀ against E. coli were 0.1 mg/mL. After treatment of E. coli (6.5 log colony-forming units/mL) with 0.1 mg/mL of CoQ₀ in LB broth for 3 h, the number of viable cells dropped below the detection limit. In addition, CoQ₀ treatment resulted in the reduction in intracellular ATP concentration, cell membrane hyperpolarization, decreased bacterial protein concentrations, and damage to cell membrane integrity and cellular morphology. These results indicated that CoQ₀ has effective antibacterial activity against E. coli, suggesting potential applications in food industry safety.