Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antimutagens against mutT.

C H Clarke, D M Shankel

    Molecular & General Genetics : MGG
    |September 9, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Certain compounds like caffeine, spermine, and quinacrine can act as antimutagens. These substances reduce mutation frequencies in E. coli mutator strains, offering insights into DNA repair mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    ARHI (DIRAS3) induces autophagy in ovarian cancer cells by downregulating the epidermal growth factor receptor, inhibiting PI3K and Ras/MAP signaling and activating the FOXo3a-mediated induction of Rab7.

    Cell death and differentiation·2014
    Same author

    Effect of diazepam and fosazepam (a soluble derivative of diazepam) on sleep in man.

    British journal of clinical pharmacology·2012
    Same author

    Antimutagenic/antioxidant activity of green tea components and related compounds.

    Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer·2004
    Same author

    The ability of certain antimutagenic agents to prevent development of antibiotic resistance.

    Mutation research·2001
    Same author

    Role of antimutagens/anticarcinogens in cancer prevention.

    BioFactors (Oxford, England)·2001
    Same author

    Does video stimulation have an important role to play in poultry behaviour research?

    British poultry science·2000
    Same journal

    A multigene family in Calothrix sp. PCC 7601 encodes phycocyanin, the major component of the cyanobacterial light-harvesting antenna

    Molecular & general genetics : MGG·2017
    Same journal

    Mutants of Schizosaccharomyces pombe which sporulate in the haploid state.

    Molecular & general genetics : MGG·2015
    Same journal

    The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats.

    Molecular & general genetics : MGG·2014
    Same journal

    Multiple mutant analysis of recombination in yeast.

    Molecular & general genetics : MGG·2012
    Same journal

    Intergenic suppression of spoO phenotypes by the Bacillus subtilis mutation rvtA.

    Molecular & general genetics : MGG·2010
    Same journal

    Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli.

    Molecular & general genetics : MGG·2001
    See all related articles

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • The mutator gene mutT- in E. coli strain 91P is known to increase mutation rates.
    • Understanding antimutagenic compounds is crucial for studying DNA stability and repair pathways.

    Purpose of the Study:

    • To investigate the antimutagenic potential of caffeine, spermine, quinacrine, and guanosine in a specific E. coli mutator strain.
    • To determine which of these compounds can effectively reduce mutation frequencies.

    Main Methods:

    • Utilizing E. coli strain 91P, which harbors the mutator gene mutT-.
    • Assessing the effect of caffeine, spermine, quinacrine, and guanosine on mutation frequency from ara- to Ara.

    Main Results:

    Related Experiment Videos

  • Caffeine, spermine, and quinacrine demonstrated significant antimutagenic activity.
  • These compounds effectively reduced the observed mutation frequencies in the E. coli mutator strain.
  • Guanosine did not exhibit any antimutagenic properties under the experimental conditions.

    • Conclusions:

    • Caffeine, spermine, and quinacrine are effective antimutagens in the context of the E. coli mutT- mutator strain.
    • The findings highlight the differential effects of various compounds on DNA mutation rates and provide a basis for further research into antimutagenesis.