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Related Experiment Videos

Vancomycin serum protein binding determination by ultrafiltration.

B H Ackerman1, E H Taylor, K M Olsen

  • 1Department of Pharmacy Practice, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock 72205.

Drug Intelligence & Clinical Pharmacy
|April 1, 1988
PubMed
Summary
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This study measured unbound and total vancomycin serum concentrations in infected patients. Vancomycin protein binding averaged 41.95%, with no membrane adsorption, aiding pharmacokinetic analysis.

Area of Science:

  • Pharmacology
  • Clinical Chemistry
  • Infectious Diseases

Background:

  • Vancomycin is a critical antibiotic for treating serious infections.
  • Accurate measurement of unbound vancomycin is crucial for effective therapeutic drug monitoring.
  • Understanding vancomycin protein binding influences its pharmacokinetic and pharmacodynamic profiles.

Purpose of the Study:

  • To quantify the protein binding of vancomycin in patient serum.
  • To establish a reliable method for measuring unbound vancomycin concentrations.
  • To correlate unbound and total vancomycin serum levels.

Main Methods:

  • Serum samples from 12 infected patients in a vancomycin pharmacokinetic study were analyzed.
  • Ultrafiltration followed by centrifugation (1000 X g, 10 min) was used to obtain ultrafiltrates.

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  • A commercial fluorescent polarization immunoassay measured both unbound and total vancomycin concentrations.

    • Main Results:

    • Observed vancomycin protein binding ranged from 7.9% to 71%.
    • The mean vancomycin protein binding was 41.95% (± 14.15%).
    • Orthogonal regression yielded the equation y = 0.597x - 0.362 (R=0.948) for unbound vs. total concentrations, with no membrane adsorption.

    Conclusions:

    • Ultrafiltration is a suitable method for determining vancomycin protein binding.
    • The established correlation aids in predicting unbound vancomycin levels from total concentrations.
    • Accurate unbound vancomycin measurements support optimized dosing strategies in clinical practice.