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Syntaxin 3 interacts with serotonin transporter and regulates its function.

Serika Motoike1, Kei Taguchi2, Kana Harada2

  • 1Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kausmi, Minami-ku, Hiroshima 734-8551, Japan; Department of Dental Anesthesiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kausmi, Minami-ku, Hiroshima 734-8551, Japan.

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|March 13, 2021
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Syntaxin-3 (STX3) interacts with the serotonin transporter (SERT). STX3 influences SERT

Keywords:
Caco-2 cellsGlycosylationMembrane traffickingSerotonin transporterSyntaxin 3

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • The serotonin transporter (SERT) is crucial for regulating serotonin levels in the brain.
  • Syntaxin-3 (STX3) is a protein involved in membrane trafficking and vesicle fusion.
  • The functional association between SERT and STX3 is not well understood.

Purpose of the Study:

  • To investigate the functional association between SERT and STX3.
  • To determine how STX3 affects SERT localization and function.
  • To explore the role of STX3 in SERT trafficking and activity in different cellular contexts.

Main Methods:

  • Overexpression of SERT and STX3 in COS-7 cells.
  • Immunoprecipitation and immunocytochemical studies to assess protein interaction and localization.
  • Functional assays measuring serotonin uptake activity.
  • STX3 knockdown experiments.
  • Studies in polarized Caco-2 cells to examine endogenous protein interactions.

Main Results:

  • STX3 directly interacts with SERT in overexpressing cells.
  • In overexpressing cells, STX3 reduces SERT uptake activity by retaining it in the ER and Golgi.
  • In polarized Caco-2 cells, STX3 is localized to microvilli and positively regulates SERT function.
  • STX3 knockdown in Caco-2 cells decreased serotonin uptake and altered SERT glycosylation.

Conclusions:

  • STX3 plays a dual role in regulating SERT function depending on the cellular context.
  • STX3 may influence SERT membrane trafficking and activity.
  • Findings suggest STX3-dependent regulation of SERT function in physiological settings.