The FlgN chaperone activates the Na⁺-driven engine of the Salmonella flagellar protein export apparatus

The bacterial flagellar protein export machinery consists of a transmembrane export gate complex and a cytoplasmic ATPase complex. The gate complex has two intrinsic and distinct H⁺-driven and Na⁺-driven engines to drive the export of flagellar structural proteins. Salmonella wild-type cells preferentially use the H⁺-driven engine under a variety of environmental conditions. To address how the Na⁺-driven engine is activated, we analyzed the fliJ(Δ13-24) fliH(Δ96-97) mutant and found that the interaction of the FlgN chaperone with FlhA activates the Na⁺-driven engine when the ATPase complex becomes non-functional. A similar activation can be observed with either of two single-residue substitutions in FlhA. Thus, it is likely that the FlgN-FlhA interaction generates a conformational change in FlhA that allows it to function as a Na⁺ channel. We propose that this type of activation would be useful for flagellar construction under conditions in which the proton motive force is severely restricted.