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Updated: Nov 13, 2025

Ground State Depletion Super-resolution Imaging in Mammalian Cells
Published on: November 5, 2017
Stimulated emission depletion microscopy for biological imaging in four dimensions: A review
Zhaoyang Wu1, Xinzhu Xu1, Peng Xi1,2
1Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
Abstract:
Stimulated emission depletion (STED) microscopy allows high lateral and axial resolution, long term imaging in living cells. Here we review recent technical advances in STED microscopy, with emphasis on resolution and measurement range of XYZt four dimensions. Different STED technical advances and novel STED probes are discussed with their respective application in biological subcellular imaging. This review may serve as a practical guide for choosing a suitable approach to the advanced STED super-resolution imaging.
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