Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

962
In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
962

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Multiscale simulation of liquid chromatography: How the mobile-phase flow velocity modulates the plate height-retention factor relationship in random and ordered beds of porous particles.

Journal of chromatography. A·2026
Same author

Recycling slalom and hydrodynamic chromatography for the improved characterization and purification of large plasmids and mRNAs in cell and gene therapy.

Journal of chromatography. A·2026
Same author

Retention modeling of oligonucleotides on an amide-based HILIC column: A descriptor-driven approach.

Journal of chromatography. A·2026
Same author

Mobile-phase selection for isomer selectivity in reversed-phase liquid chromatography: Evaluation of conventional and green, binary and ternary mobile phases for the separation of carvacrol and thymol.

Journal of chromatography. A·2026
Same author

The role of particle size distribution on permeability and efficiency of slurry-packed liquid chromatography columns.

Journal of chromatography. A·2026
Same author

Molecular dynamics simulations of mixed-mode chromatography: Generation and solvation of a silica-based, reversed-phase/anion-exchange stationary phase.

Journal of chromatography. A·2026

Related Experiment Video

Updated: Nov 13, 2025

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

517

Multiple-open-tubular column enabling transverse diffusion. Part 2: Channel size distribution and structure

Fabrice Gritti1, Dzmitry Hlushkou2, Ulrich Tallarek2

  • 1Waters Corporation, Instrument/Core Research/Fundamental, 34 Maple Street, Milford, MA, 01757, USA.

Journal of Chromatography. A
|March 14, 2021
PubMed
Summary
This summary is machine-generated.

This study models band broadening in multiple-open-tubular columns enabling transverse diffusion (MOTTD). Channel diameter variations moderately impact performance, but significantly affect analysis speed when diffusion is slow.

Keywords:
Column technologyDesign optimizationGeneral model of band broadeningMultiple-open-tubular columnSize distribution of flow-through channelsspeed-resolution performance

More Related Videos

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

9.8K
The Diffusion of Passive Tracers in Laminar Shear Flow
08:01

The Diffusion of Passive Tracers in Laminar Shear Flow

Published on: May 1, 2018

8.9K

Related Experiment Videos

Last Updated: Nov 13, 2025

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

517
A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

9.8K
The Diffusion of Passive Tracers in Laminar Shear Flow
08:01

The Diffusion of Passive Tracers in Laminar Shear Flow

Published on: May 1, 2018

8.9K

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • Multiple-open-tubular columns enabling transverse diffusion (MOTTD) offer unique separation capabilities.
  • Previous work established a model for band broadening in MOTTD columns, considering longitudinal diffusion, velocity bias, and mass transfer.
  • Further investigation is needed to incorporate the effects of inter-channel velocity biases due to channel diameter variations.

Purpose of the Study:

  • To complete the band broadening model for MOTTD columns by including the impact of short-range inter-channel velocity biases.
  • To analyze the influence of channel diameter distribution (relative standard deviation, RSD) on MOTTD column performance.
  • To determine optimal column parameters for balancing analysis speed and separation efficiency.

Main Methods:

  • Developed a comprehensive model for band broadening in MOTTD columns, incorporating channel diameter distribution.
  • Validated the model using data from the lattice-Boltzmann method for fluid flow and a random-walk particle-tracking technique for transport.
  • Simulated various ratios of channel diameter to distance (ρ) and relative standard deviations (RSD) of channel diameters.

Main Results:

  • The relative standard deviation of channel diameters has a moderate impact (≤30% increase in minimum plate height) on the optimum plate number.
  • Plate height can increase by up to 100% at high velocities when analyte mass transfer is limited by slow transverse diffusion.
  • Fastest analyses are achieved with small ρ values, while longest analysis times require large ρ values for maximum performance.

Conclusions:

  • The completed model provides a more accurate prediction of band broadening in MOTTD columns considering real-world channel variations.
  • Channel diameter variability has a manageable effect on optimal column performance but can significantly influence analysis speed under specific conditions.
  • Design choices regarding the structural parameter ρ are crucial for optimizing MOTTD columns for either rapid analysis or maximum separation efficiency.