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Related Concept Videos

Erythropoiesis01:14

Erythropoiesis

5.3K
Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Factors Affecting Erythropoiesis01:24

Factors Affecting Erythropoiesis

4.5K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
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Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

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Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
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Related Experiment Video

Updated: Nov 13, 2025

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

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Proteomic/transcriptomic analysis of erythropoiesis.

Marjorie Brand1,2, Jeffrey A Ranish3

  • 1Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute.

Current Opinion in Hematology
|March 14, 2021
PubMed
Summary

New proteomic studies reveal crucial posttranscriptional regulation in red blood cell development (erythropoiesis). Integrating proteomic data into gene regulatory networks (GRNs) is essential for a comprehensive understanding of this complex process.

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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
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Related Experiment Videos

Last Updated: Nov 13, 2025

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

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Area of Science:

  • Hematology
  • Molecular Biology
  • Systems Biology

Background:

  • Erythropoiesis is a complex, hierarchical process where hematopoietic stem cells differentiate into red blood cells.
  • Gene regulatory networks (GRNs) are critical for understanding cellular responses to environmental signals during differentiation.
  • Traditionally, GRNs were derived from transcriptomic data, but recent findings emphasize posttranscriptional regulation.

Purpose of the Study:

  • To review recent proteomic studies enhancing the understanding of erythropoiesis.
  • To focus on quantitative mass spectrometry in measuring protein factors during erythroid differentiation.
  • To highlight challenges and future prospects in integrating multi-omics data for predictive erythropoiesis models.

Main Methods:

  • Review of recent proteomic studies.
  • Focus on quantitative mass spectrometry for protein abundance measurement.
  • Discussion of challenges in integrating transcriptomic, proteomic, and other omics data.

Main Results:

  • Proteomic studies have significantly expanded knowledge of erythropoiesis beyond transcriptomics.
  • Posttranscriptional mechanisms play a major role in gene expression regulation during erythropoiesis.
  • Quantitative mass spectrometry provides insights into transcription factor and cofactor dynamics.

Conclusions:

  • Integrating proteomic data into GRNs is crucial for a refined understanding of erythropoiesis.
  • Developing predictive models requires integrating multiple layers of biological data (multi-omics).
  • Single-cell proteomics offers a promising future direction for studying erythroid differentiation.