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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Lb2Cas12a and its engineered variants mediate genome editing in human cells

Xiaoyu Liu1, Li Lin1, Lianchao Tang1

  • 1School of Ophthalmology and Optometry, Eye Hospital, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|March 14, 2021
PubMed
Summary

Researchers discovered Lb2Cas12a, a novel Cas12a enzyme with enhanced genome editing capabilities in human cells. This new tool offers greater PAM flexibility and expanded target selection for precision gene editing applications.

Keywords:
PAMRS1activityfidelity

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