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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

647
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
647

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Image-guided Raman spectroscopy probe-tracking for tumor margin delineation.

Conor C Horgan1,2,3, Mads S Bergholt1,2,3, May Zaw Thin4

  • 1Imperial College London, Department of Materials, London, United Kingdom.

Journal of Biomedical Optics
|March 14, 2021
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This study introduces an image-guided Raman spectroscopy system for real-time tumor margin delineation. Combining probe tracking and fluorescence imaging enhances precision in surgical resections.

Keywords:
Raman spectroscopycancerfluorescence guidancemargin delineationprobe tracking

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Area of Science:

  • Medical imaging
  • Spectroscopy
  • Computer vision

Background:

  • Accurate tumor margin delineation is critical for successful cancer surgery, yet positive margin rates remain high.
  • Raman spectroscopy offers high accuracy for tumor detection but is limited by pointwise application for margin delineation.

Purpose of the Study:

  • To develop a multimodal computer vision-based system for enhanced tumor detection and margin delineation.
  • To extend Raman spectroscopic diagnostics for precise identification and outlining of cancerous tissues.

Main Methods:

  • Real-time tumor margin delineation using visual tracking of a Raman spectroscopic probe.
  • Integration with protoporphyrin IX fluorescence imaging for fluorescence-guided margin delineation.

Main Results:

  • The system achieved real-time Raman spectroscopic tumor margin delineation on ex vivo human tumor biopsies and an in vivo mouse model.
  • Protoporphyrin IX fluorescence imaging enabled fluorescence-guided Raman spectroscopic margin delineation in a tissue phantom.

Conclusions:

  • The image-guided Raman spectroscopic probe-tracking system facilitates tumor margin delineation.
  • Compatibility with white light and fluorescence imaging shows potential for clinical tumor resection surgeries.