Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Nov 13, 2025

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Published on: February 2, 2024

Rolling Circle Replication for Biosensing, Bioimaging, and Biomedicine.

Shuzhen Yue¹, Yuwei Li¹, Zhenjie Qiao¹

¹College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, PR China.

Trends in Biotechnology

|March 15, 2021

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Topology-controllable DNA framework-based nanocapsule enhances tumor suppression via adaptive modulation of lysosomal-mitochondrial axis.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

A Biomimetic Nanozyme Engineered via Coordination and Confinement for Photothermal-Reinforced Catalytic Detection of Extracellular Vesicles and Diagnosis of Cancer.

Analytical chemistry·2026

Same author

Nucleic Acid Nanotechnology-Empowered CRISPR-Cas12a Systems for Biosensing and Bioimaging Applications.

Small methods·2026

Same author

Multifunctional CuS-CDs@MnO<sub>2</sub> nanoparticles and liposome-assisted amplification for triple-mode detection of enrofloxacin.

Analytical methods : advancing methods and applications·2026

Same author

Self-powered PEC/EC dual-mode sensing platform for sensitive TNF-α detection based on multifunctional mesoporous silica nanospheres.

Analytical methods : advancing methods and applications·2026

Same author

Fluorescent aptasensor for enrofloxacin detection based on APE1-assisted amplification and CsPbBr<sub>3</sub>@MSN nanocomposites.

The Analyst·2026

Rolling circle replication (RCR) is a versatile isothermal enzymatic reaction. This powerful biosensing tool offers high amplification efficiency for detecting biomolecules and has applications in bioimaging and drug delivery.

Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

Rolling circle replication (RCR) is an isothermal enzymatic reaction.
RCR encompasses rolling circle amplification (RCA) and rolling circle transcription (RCT).
RCR is recognized for its high amplification efficiency.

Purpose of the Study:

To introduce the fundamentals of Rolling Circle Replication (RCR).
To review recent advances in RCR applications.
To discuss challenges and opportunities in RCR-based biotechnology.

Main Methods:

Literature review of RCR fundamentals.
Analysis of recent RCR-based detection methods.
Evaluation of RCR applications in biosensing, bioimaging, and biomedicine.

Keywords:

DNA/RNA structures bioimaging biomedicine biosensing rolling circle amplification rolling circle transcription

More Related Videos

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Related Experiment Videos

Last Updated: Nov 13, 2025

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Published on: February 2, 2024

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Main Results:

RCR is a highly efficient isothermal enzymatic reaction.
RCR serves as a potent tool for biomolecule detection.
RCR has expanded applications in bioimaging and drug delivery systems.

Conclusions:

RCR is a valuable technique with diverse applications.
Recent advances highlight RCR's potential in biosensing, bioimaging, and biomedicine.
Further research into RCR-based biotechnology promises significant advancements.