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Related Experiment Video

Updated: Nov 13, 2025

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
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Wdhd1 is essential for early mouse embryogenesis.

Hsiang-Hsuan Fan1, Kuo-Hong Lee1, You-Tzung Chen2

  • 1Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei 100, Taiwan.

Biochimica Et Biophysica Acta. Molecular Cell Research
|March 15, 2021
PubMed
Summary
This summary is machine-generated.

WD repeat and HMG-box DNA binding protein 1 (Wdhd1) is crucial for mouse embryonic cell proliferation. Wdhd1 deficiency in homozygous null embryos severely impacts growth and viability, highlighting its essential in vivo role.

Keywords:
CRISPR/Cas9DNA replicationEmbryogenesisMouseProliferationWdhd1

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • WD repeat and HMG-box DNA binding protein 1 (Wdhd1) is essential for DNA replication initiation.
  • Previous studies suggest Wdhd1's role in DNA synthesis across various model organisms.
  • In vivo functional data for mammalian Wdhd1 during embryogenesis is lacking.

Purpose of the Study:

  • To investigate the in vivo function of Wdhd1 during mouse embryogenesis.
  • To generate and characterize a Wdhd1 null mouse model.

Main Methods:

  • CRISPR/Cas9 genome editing to create Wdhd1 null mice.
  • Quantitative reverse-transcription polymerase chain reaction for Wdhd1 expression analysis.
  • Histological assessment of cell proliferation in pre- and peri-implantation embryos.

Main Results:

  • Wdhd1 heterozygous mice are normal and fertile.
  • Wdhd1 homozygous null embryos exhibit reduced cell proliferation by the gastrulation stage.
  • Homozygous null embryos show severely hampered growth and viability.

Conclusions:

  • Wdhd1 is essential for efficient cell proliferation during mouse embryogenesis.
  • The study establishes a critical in vivo role for Wdhd1 in mammalian development.