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Updated: Nov 13, 2025

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Published on: June 21, 2018
Cover Feature: Regulation of pyruvate dehydrogenase complex related to lactate switch in CHO cells
Abstract:
DOI: 10.1002/elsc.202000037 The cover feature visualizes our recent article about the investigation of the regulation of the Pyruvate Dehydrogenase Complex (PDC) during the lactate switch in batch cultures of Chinese Hamster Ovary cells. The relevance of this work to bioprocess engineering is highlighted in the background and the central cellular metabolic regulations are shown symbolically on the right-hand side. The regulation of PDC through phosphorylation was quantified at three regulating sites using a novel indirect flow cytometry protocol, shown as "glowing" antibodies. For details see article DOI 10.1002/elsc.202000037 on page 99.
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