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Related Experiment Video

Updated: Nov 13, 2025

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

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Tracking Eye Movements During Sleep in Mice.

Qingshuo Meng1, Xinrong Tan1, Chengyong Jiang1

  • 1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai, China.

Frontiers in Neuroscience
|March 15, 2021
PubMed
Summary

Researchers developed a new device to track eye movements in mice during sleep. This technology distinguishes between tonic and phasic rapid eye movement (REM) sleep stages, offering insights into brain activity during sleep.

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Area of Science:

  • Neuroscience
  • Sleep Science
  • Animal Models

Background:

  • Eye movements reflect cognitive status and show similarities between wakefulness and REM sleep.
  • REM sleep in humans is categorized into tonic and phasic stages based on eye movement frequency.
  • Mice are crucial animal models for sleep research, but REM sleep stages are not well-defined in this species.

Purpose of the Study:

  • To develop and validate a novel device for simultaneous recording of EEG, EMG, and eye movements in mice.
  • To investigate eye movement patterns during sleep-wake cycles in mice.
  • To determine if REM sleep can be sub-divided into tonic and phasic stages in mice based on eye movement characteristics.

Main Methods:

  • Development of a device using a subconjunctival magnet and magnetic sensor for eye movement tracking.
Keywords:
biocompatibilityeye movement trackingphasic REMsleep–wake cycletonic REM

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Related Experiment Videos

Last Updated: Nov 13, 2025

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
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Video-oculography in Mice
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  • Simultaneous recording of electroencephalogram (EEG) and electromyogram (EMG) alongside eye movements.
  • Validation of magnetic eye tracking against video-oculography and assessment of biocompatibility.

    • Main Results:

    • The developed magnetic eye tracking system demonstrated high correlation with video-oculography.
    • The subconjunctival magnet implant showed good biocompatibility in mice.
    • Phasic REM sleep in mice was characterized by higher 0.50 Hz oscillation power and lower power in the 1.50-7.25 Hz and 9.50-12.00 Hz ranges compared to tonic REM sleep.

    Conclusions:

    • The novel device enables simultaneous, high-resolution recording of EEG, EMG, and eye movements in mice.
    • This technology facilitates the distinction between tonic and phasic REM sleep in mice.
    • The findings provide a foundation for further research into the neurobiological mechanisms of sleep stages in mice.