Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative electroencephalography as a tool for evaluating pain-related neurophysiologic responses in cattle: a systematic review.

American journal of veterinary research·2026
Same author

Impact of climate, air pollution, and urbanization on chronic respiratory infections.

Expert review of respiratory medicine·2026
Same author

Vertebral artery contribution to cerebral cortex perfusion in cattle after slaughter by ventral neck incision: a systematic review.

Frontiers in veterinary science·2026
Same author

Co-simulation framework combining a microscopically detailed point neuron model of the hippocampal CA1 region with the macroscopic high-resolution virtual brain model.

Journal of computational neuroscience·2026
Same author

A network of Bayesian agents for reward prediction and noise tolerance.

iScience·2025
Same author

A Computational Model of the Respiratory CPG for the Artificial Control of Breathing.

Bioengineering (Basel, Switzerland)·2025

Related Experiment Video

Updated: Nov 13, 2025

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
09:30

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

2.7K

Real Time Generation of Three Dimensional Patterns for Multiphoton Stimulation.

Paolo Pozzi1, Jonathan Mapelli1,2

  • 1Department of Beiomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy.

Frontiers in Cellular Neuroscience
|March 15, 2021
PubMed
Summary

We developed a faster method for optogenetics, enabling high-quality 3D neuron stimulation at video rates. This breakthrough in neuroscience accelerates experiments and unlocks new possibilities for brain research.

Keywords:
GPU (CUDA)computer generated hologramsmultiphoton microcopyoptogeneticsspatial light modulatorswavefront control

More Related Videos

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
09:30

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

16.0K
Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions
07:38

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

1.9K

Related Experiment Videos

Last Updated: Nov 13, 2025

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
09:30

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

2.7K
Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
09:30

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

16.0K
Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions
07:38

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

1.9K

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Engineering

Background:

  • Optogenetics revolutionizes neuroscience by enabling targeted neuronal stimulation in 3D.
  • Multiphoton excitation combined with optogenetics allows precise neuronal targeting.
  • Current methods using spatial light modulation (SLM) are limited by slow computational times for 3D pattern generation.

Purpose of the Study:

  • To implement a compressed sensing Gerchberg-Saxton algorithm on a GPU for rapid, high-quality 3D optogenetic pattern generation.
  • To overcome the computational limitations of existing SLM-based methods.
  • To enable advanced neuroscience applications requiring real-time neuronal control.

Main Methods:

  • Implementation of a compressed sensing Gerchberg-Saxton algorithm.
  • Utilizing a consumer graphical processing unit (GPU) for accelerated computation.
  • Generating 3D excitation point clouds for neuronal stimulation.

Main Results:

  • Achieved high-quality 3D pattern generation at video rate.
  • Significantly reduced computational time for pattern calculation.
  • Overcame the trade-off between pattern quality and update speed in SLM systems.

Conclusions:

  • The GPU-accelerated algorithm dramatically reduces experimental dead times in optogenetics.
  • Enables previously impossible applications, such as feedback-controlled neuronal network activity.
  • Facilitates real-time compensation of motion artifacts during imaging and stimulation.