Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biodegradable Alginate-Chitosan Hollow Nanospheres for Codelivery of Doxorubicin and Paclitaxel for the Effect of Human Lung Cancer A549 Cells.

BioMed research international·2018
Same author

[SHORT-TERM EFFECTIVENESS OF PERCUTANEOUS PEDICLE SCREW GUIDED WITH PHOTOELECTRIC NAVIGATION FOR THORACOLUMBAR FRACTURES].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2018
Same author

Epigenetic dysregulation of ZEB1 is involved in LMO2-promoted T-cell acute lymphoblastic leukaemia leukaemogenesis.

Biochimica et biophysica acta. Molecular basis of disease·2018
Same author

Theoretical study of the intermolecular recognition mechanism between Survivin and substrate based on conserved binding mode analysis.

Journal of molecular graphics & modelling·2018
Same author

The asymmetry of neural symptoms in Wilson's disease patients detecting by diffusion tensor imaging, resting-state functional MRI, and susceptibility-weighted imaging.

Brain and behavior·2018
Same author

Estimates of abundance and diversity of Shewanella genus in natural and engineered aqueous environments with newly designed primers.

The Science of the total environment·2018
Same journal

Editorial: Association of diabetes mellitus with cognitive impairment and neurological disorders, volume II.

Frontiers in endocrinology·2026
Same journal

Free water-mediated associations among choroid plexus enlargement, white matter lesions, and cognitive performance in type 2 diabetes mellitus.

Frontiers in endocrinology·2026
Same journal

Development and validation of a uric acid-inflammation-metabolism score for predicting osteoarthritis risk: evidence from NHANES 2007-2018 and an external Chinese cohort.

Frontiers in endocrinology·2026
Same journal

Sinonasal and skull base phosphaturic mesenchymal tumours: a case series and narrative review.

Frontiers in endocrinology·2026
Same journal

The double-edged sword effect of estrogen in kidney disease and its precision modulation.

Frontiers in endocrinology·2026
Same journal

Cholesterol, high-density lipoprotein, and glucose index as a cardiometabolic marker associated with heart rate variability and 1-year cardiovascular rehospitalization in chronic coronary syndromes with comorbid anxiety: a retrospective cohort study.

Frontiers in endocrinology·2026
See all related articles

Related Experiment Video

Updated: Nov 13, 2025

An Ex vivo Culture System to Study Thyroid Development
08:33

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

11.9K

Using Single-Cell RNA-Seq Data to Trace Tissue Cells Responsive to Thyroid Hormones.

Liang Hu1, Chao Wu2

  • 1Department of Thyroid Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Frontiers in Endocrinology
|March 15, 2021
PubMed
Summary
This summary is machine-generated.

Thyroid hormones regulate bodily functions via THRA and THRB receptors. This study uses single-cell RNA sequencing to identify specific cell types sensitive to thyroid hormone action across various tissues.

Keywords:
Thra/Thrbbioinformatics analysiscell-type-specific expressionsingle-cell RNA-Seqthyroid hormone

More Related Videos

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

2.4K
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

25.9K

Related Experiment Videos

Last Updated: Nov 13, 2025

An Ex vivo Culture System to Study Thyroid Development
08:33

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

11.9K
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

2.4K
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

25.9K

Area of Science:

  • Endocrinology
  • Genomics
  • Computational Biology

Background:

  • Thyroid hormones are crucial for numerous physiological processes, acting through thyroid hormone receptors alpha (THRA) and beta (THRB).
  • Cellular heterogeneity within tissues leads to variable responses to thyroid hormones, complicating the understanding of their regulatory mechanisms.
  • Identifying specific responsive cell types is vital for elucidating the molecular basis of thyroid hormone-related diseases.

Purpose of the Study:

  • To apply single-cell RNA sequencing (scRNA-Seq) data from the Tabula Muris Consortium to precisely identify thyroid hormone-responsive cells within various tissues.
  • To develop and demonstrate computational approaches for analyzing scRNA-Seq data to pinpoint cell types and subtypes with differential expression of THRA and THRB.

Main Methods:

  • Utilized scRNA-Seq data from the Tabula Muris Consortium, encompassing 23 tissues from *Mus musculus*.
  • Calculated cell type-specific expression of Thra and Thrb to identify potentially sensitive cell populations.
  • Employed cell clustering algorithms to detect subtypes with low Thra or Thrb expression, indicating potential responsiveness.
  • Validated the approach by tracing responsive cell types in liver tissue treated with thyroid hormones.

Main Results:

  • Successfully quantified Thra and Thrb expression across diverse cell types and tissues.
  • Identified specific cell subtypes with potentially altered thyroid hormone sensitivity based on receptor expression levels.
  • Demonstrated the successful tracing of thyroid hormone-responsive cells in liver tissue using scRNA-Seq data.

Conclusions:

  • Single-cell RNA sequencing provides a powerful resolution to map thyroid hormone-responsive cells within complex tissues.
  • The developed computational methods offer a valuable framework for identifying cell-type-specific responses to thyroid hormones.
  • These findings provide crucial insights for future wet-lab investigations into thyroid hormone regulation and associated pathologies.