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miR-146b correlates with increased disease activity and psoriatic tissue inflammation and promotes keratinocyte

Ling Zhang1, Shenglan Zhang2, Jianbin Wang1

  • 1Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056002, P.R. China.

Experimental and Therapeutic Medicine
|March 15, 2021
PubMed

Insights

MicroRNA (miR)-146b is elevated in psoriasis, correlating with disease severity and inflammation. Overexpression of miR-146b promotes keratinocyte proliferation and inhibits apoptosis in psoriasis.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Immunology

Background:

  • Psoriasis is a chronic inflammatory skin disease.
  • MicroRNAs play crucial roles in regulating cellular processes and disease pathogenesis.
  • The specific role of microRNA (miR)-146b in psoriasis remains to be fully elucidated.

Purpose of the Study:

  • To investigate the expression levels of miR-146b in psoriatic skin tissue.
  • To determine the correlation between miR-146b expression and psoriasis disease activity and inflammation.
  • To explore the effects of miR-146b on keratinocyte proliferation and apoptosis in vitro.

Main Methods:

  • Quantification of miR-146b expression in 110 patients' psoriatic and unaffected skin using reverse transcription-quantitative PCR (RT-qPCR).
  • Assessment of disease activity using Psoriasis Area and Severity Index (PASI) scores and body surface area.
  • In vitro study involving transfection of HaCaT cells with miR-146b overexpression plasmids to analyze cell proliferation and apoptosis.

Main Results:

  • miR-146b expression was significantly increased in psoriatic tissue compared to unaffected tissue.
  • miR-146b levels showed a positive correlation with PASI scores and inflammatory markers (TNF-α, IL-6, IL-17).
  • Overexpression of miR-146b in HaCaT cells promoted proliferation and inhibited apoptosis, including TRAIL-induced apoptosis.

Conclusions:

  • miR-146b is upregulated in psoriasis and is associated with disease activity and inflammation.
  • miR-146b may contribute to the pathogenesis of psoriasis by promoting keratinocyte proliferation and inhibiting apoptosis.
Keywords:
disease activityinflammationkeratinocytemicroRNA-146bpsoriasis

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