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Updated: Nov 12, 2025

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Published on: June 26, 2019
Integrative transcription start site identification with iTiSS
Christopher S Jürges1, Lars Dölken1, Florian Erhard1
1Institute for Virology and Immunobiology, Julius-Maximilians-University Würzburg, Würzburg 97078, Germany.
Summary:
Many experimental approaches have been developed to identify transcription start sites (TSS) from genomic scale data. However, experiment specific biases lead to large numbers of false-positive calls. Here, we present our integrative approach iTiSS, which is an accurate and generic TSS caller for any TSS profiling experiment in eukaryotes, and substantially reduces the number of false positives by a joint analysis of several complementary datasets.
Availability And Implementation:
iTiSS is platform independent and implemented in Java (v1.8) and is freely available at https://www.erhard-lab.de/software and https://github.com/erhard-lab/iTiSS.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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