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Chemokine ligand 28 (CCL28) negatively regulates trabecular bone mass by suppressing osteoblast and osteoclast

Rina Iwamoto1, Takumi Takahashi2, Kazuto Yoshimi3

  • 1Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.

Journal of Bone and Mineral Metabolism
|March 15, 2021
PubMed
Abstract

Insights

Chemokine ligand 28 (CCL28) negatively regulates bone mass by suppressing osteoblast and osteoclast activity. This finding offers new therapeutic targets for osteoporosis and bone metabolism diseases.

Area of Science:

  • Bone biology
  • Immunology
  • Metabolic diseases

Background:

  • Bone metabolism imbalances contribute to age-related diseases like osteoporosis.
  • While some chemokines are implicated in bone regulation, many remain unstudied.
  • The role of chemokine ligand 28 (CCL28) in bone metabolism requires investigation.

Purpose of the Study:

  • To elucidate the function of CCL28 in regulating bone metabolism.
  • To determine the impact of CCL28 deficiency on bone mass and cellular activity.

Main Methods:

  • Analysis of wild-type and Ccl28 knockout mice using microcomputed tomography and bone morphometry.
  • Immunohistochemistry to localize CCL28 in bone tissue.
  • Enzyme-linked immunosorbent assay and qRT-PCR for osteoblast and osteoclast markers.
  • In vitro studies with MC3T3-E1 and bone marrow macrophages to assess direct CCL28 effects.

Main Results:

  • Ccl28 deficiency significantly increased bone mass and osteoblast numbers.
  • CCL28 was localized to osteoblasts and osteoclasts.
  • Ccl28 deficiency enhanced osteoblast and osteoclast maturation.
  • CCL28 treatment inhibited osteoblast and osteoclast activity without affecting differentiation.

Conclusions:

  • CCL28 acts as a negative regulator of bone mass by inhibiting osteoblast and osteoclast activity.
  • These findings contribute to understanding bone immunology.
  • CCL28 presents a potential target for novel osteoporosis treatments.
Keywords:
Bone metabolismChemokine ligand 28OsteoblastOsteoclast

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