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Autographa Californica Multiple Nucleopolyhedrovirus orf13 Is Required for Efficient Nuclear Egress of Nucleocapsids

Xingang Chen1,2, Xiaoqin Yang1,2, Chengfeng Lei1

  • 1Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.

Virologica Sinica
|March 15, 2021
PubMed

Insights

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) orf13 (ac13) is essential for efficient nucleocapsid nuclear egress and virion budding. Deleting ac13 significantly reduces viral budding but does not impact occlusion body formation.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Autographa californica multiple nucleopolyhedrovirus (AcMNPV) orf13 (ac13) is a conserved gene in alphabaculoviruses with an unknown function.
  • Understanding viral gene function is crucial for baculovirus research and applications.

Purpose of the Study:

  • To investigate the function of the AcMNPV orf13 gene in the viral life cycle.
  • To determine the role of ac13 in viral replication, assembly, and egress.

Main Methods:

  • Gene deletion analysis of ac13 in AcMNPV.
  • Localization studies of the ac13-encoded protein.
  • Analysis of viral genome replication, nucleocapsid assembly, virion budding, and occlusion body formation.

Main Results:

  • AcMNPV ac13 is a late gene encoding a protein that localizes to the nuclear lamina.
  • Deletion of ac13 did not affect viral genome replication, nucleocapsid assembly, or occlusion body formation.
  • ac13 deletion reduced virion budding by approximately 400-fold and impaired nuclear egress of nucleocapsids.

Conclusions:

  • AcMNPV orf13 is required for efficient nuclear egress of nucleocapsids during virion budding.
  • The ac13 gene is dispensable for occlusion body formation.
  • AcMNPV ac13 plays a critical role in the late stages of the viral life cycle, specifically in virion egress.
Keywords:
Autographa californica multiple nucleopolyhedrovirus (AcMNPV)Nucleocapsid egressOB morphogenesisorf13

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