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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
666

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A pH-responsive fluorescent sensor based on a new pyranoxanthylium salt.

Vânia Gomes1, Nuno Mateus1, Victor de Freitas1

  • 1REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade Do Porto, Rua Do Campo Alegre, 687, 4169-007, Porto, Portugal.

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|March 15, 2021
PubMed
Summary

A new pH-responsive fluorescent dye was synthesized. This xanthylium-derived pigment exhibits selective "off-on-off" fluorescence between pH 5 and 9, making it ideal for biological sensing applications.

Keywords:
Fluorescent probesSensorsThermodynamicsUV–Vis spectroscopyYellow dyepH-stimuli

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Area of Science:

  • * Organic Chemistry
  • * Photophysics
  • * Materials Science

Background:

  • * Stimuli-responsive pigments are valuable for technological applications, including colorants, sensors, and fluorescent probes.
  • * Xanthylium dyes are a class of compounds with potential for tunable optical properties.

Purpose of the Study:

  • * To synthesize a novel xanthylium-derived pigment.
  • * To investigate the chemical and photophysical properties of the synthesized dye in hydroalcoholic solutions across a range of pH values.
  • * To evaluate its potential as a pH-responsive fluorescent probe.

Main Methods:

  • * Synthesis of a new xanthylium-derived pigment.
  • * UV-Vis and fluorescence spectroscopy were employed to study the pigment's properties.
  • * UV-Vis titration was performed to determine acidic constants (pKa) and identify different chemical species.

Main Results:

  • * Four distinct colored chemical species (AH+, A, A-, and A2-) were identified in hydroalcoholic solution between pH 1 and 12.
  • * Thermodynamic acidic constants were determined: pKa1 = 4.80 ± 0.03, pKa2 = 6.51 ± 0.05, and pKa3 = 8.64 ± 0.01.
  • * The dye exhibited pH-dependent fluorescence, with significant emission observed for the anionic quinoidal base (A-) between pH 5 and 9, peaking at pH 7.5 (λex 467 nm/λem 510 nm).

Conclusions:

  • * The synthesized xanthylium dye demonstrates tunable photophysical properties in response to pH changes.
  • * Its selective fluorescence emission in the pH range of 5-9, with maximum intensity at pH 7.5, highlights its potential.
  • * The dye is a promising candidate for an
  • off-on-off
  • pH-responsive fluorescent probe for biological and biomedical applications.