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Jasmonic acid-responsive RRTF1 transcription factor controls DTX18 gene expression in hydroxycinnamic acid amide
Jinbo Li1,2, Yu Meng1,3, Kaixuan Zhang1
1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plant Physiology
|March 15, 2021
Summary
Jasmonates regulate plant defense by controlling hydroxycinnamic acid amide (HCAA) production. This study identifies RRTF1 as a key transcription factor that enhances HCAA secretion and plant resistance to fungal pathogens.
Area of Science:
- Plant molecular biology
- Plant hormone signaling
- Plant-pathogen interactions
Background:
- Jasmonates (JAs) are crucial plant hormones regulating secondary metabolite biosynthesis, including hydroxycinnamic acid amides (HCAAs), vital for plant defense.
- The DTX18 transporter facilitates HCAA accumulation at the plant surface, but its gene regulation by transcription factors remains unclear.
Purpose of the Study:
- To elucidate the regulatory mechanism of DTX18 gene expression by jasmonic acid-responsive transcription factors.
- To identify key regulators involved in HCAA-mediated plant defense.
Main Methods:
- Yeast one-hybrid and two-hybrid screening to identify interacting proteins.
- Gene expression analysis and mutant studies in plants.
- Protein degradation assays via the 26S proteasome pathway.
Main Results:
- Redox-responsive TF 1 (RRTF1) was identified as a positive regulator of DTX18 transcription, promoting HCAA secretion and enhancing resistance to Botrytis cinerea.
- BPM1 and BPM3 proteins interact with RRTF1, mediating its degradation via the 26S proteasome pathway.
- Jasmonic acid stabilizes RRTF1, while knockout of BPM1/BPM3 enhances RRTF1 accumulation and plant defense.
Conclusions:
- RRTF1 acts as a critical mediator in JA-induced HCAA accumulation and plant defense.
- The RRTF1-BPM interaction fine-tunes RRTF1 stability and DTX18 expression, impacting plant resistance to fungal pathogens.
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