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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Updated: Nov 12, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Applications of Single-Cell DNA Sequencing.

Gilad D Evrony1, Anjali Gupta Hinch2, Chongyuan Luo3

  • 1Center for Human Genetics and Genomics, Grossman School of Medicine, New York University, New York, NY 10016, USA;

Annual Review of Genomics and Human Genetics
|March 16, 2021
PubMed
Summary

Single-cell DNA sequencing unlocks new biological insights and biomedical applications. This technology is crucial for understanding complex processes like somatic mutagenesis and has future potential in oncology and fertility.

Keywords:
DNA modificationsgerm cellslineage tracingorganismal developmentsingle-cell DNA sequencingsingle-cell genomics

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Genomic analyses of single cells have emerged as a powerful tool over the past decade.
  • Single-cell DNA sequencing addresses fundamental biological questions previously inaccessible.
  • While single-cell RNA and protein profiling advanced cellular diversity studies, DNA analysis remains critical for many applications.

Purpose of the Study:

  • To review the diverse applications of single-cell DNA sequencing.
  • To highlight biological questions uniquely addressed by single-cell DNA sequencing.
  • To discuss the future impact of advancing single-cell DNA sequencing technologies in various fields.

Main Methods:

  • Review of existing literature and technological advancements in single-cell DNA sequencing.
  • Analysis of current and potential applications across different research domains.
  • Discussion of the unique suitability of single-cell DNA sequencing for specific biological inquiries.

Main Results:

  • Single-cell DNA sequencing provides critical insights into somatic mutagenesis, organismal development, genome function, and microbiology.
  • The technology is poised for significant biomedical and clinical impact, particularly in oncology and fertility.
  • It offers unique advantages over other single-cell profiling methods for certain research questions.

Conclusions:

  • Single-cell DNA sequencing is indispensable for answering fundamental biological questions and driving biomedical innovation.
  • Continued technological advancements will broaden the scope and impact of single-cell DNA sequencing.
  • This technique is essential for future research in diverse areas from basic biology to clinical diagnostics.