Fast Identification of Food Thickeners by Nontargeted NMR-Spectroscopy

Food thickeners are carbohydrate additives that can only be determined by long-term, multistep analysis. Fast methods to directly determine thickeners in food matrixes are therefore welcome. In this study, a rapid procedure based on the direct ¹H NMR analysis of food samples dissolved in deuterated water was developed. Individual thickeners were assigned due to specific marker signals gleaned from two-dimensional NMR analyses. The combination of one-dimensional ¹H NMR and DOSY experiments enabled unequivocal assignments of thickeners even in complex matrixes. Using this approach, gum arabic, carrageenan, agar-agar, galactomannans, and pectin could be identified in pastille, glaze, and fruit spread. Because of low concentrations (<0.5%-1%, w/w), the same thickeners and others such as xanthan gum and alginate could not be determined directly by NMR in curry sauce, rice pudding, choco milk drink, and lemon peel flavor. Moreover, NMR analyses of the hydrolysate did not reveal the specific monomeric units of the thickeners under study, as shown for the hydrolysate of lemon peel flavor. Nevertheless, the NMR approach could provide welcome means in the future to directly determine intact thickeners in food.