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Related Experiment Videos

Phosphate transport in intestinal brush-border membrane.

S P Shirazi-Beechey1, J P Gorvel, R B Beechey

  • 1Department of Biochemistry, University College of Wales, Aberystwyth, UK.

Journal of Bioenergetics and Biomembranes
|April 1, 1988
PubMed
Summary

Phosphate uptake in rabbit small intestine is sodium-dependent and occurs in the duodenum. Antibodies targeting jejunum and ileum enhanced this transport, while duodenal antibodies inhibited it.

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Area of Science:

  • Gastroenterology
  • Cellular Physiology
  • Molecular Biology

Background:

  • Phosphate is crucial for cellular functions and its absorption is tightly regulated.
  • The small intestine, particularly the duodenum, is a primary site for phosphate absorption.
  • Understanding the molecular mechanisms of phosphate transport is essential for managing phosphate homeostasis.

Purpose of the Study:

  • To elucidate the mechanism of sodium-dependent phosphate uptake in rabbit duodenal enterocytes.
  • To investigate the role of intravesicular ions and membrane-permeable anions in phosphate transport.
  • To characterize the effect of antibodies against intestinal membrane vesicles on phosphate transport.

Main Methods:

  • Vesicle preparations from rabbit small intestine.

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  • Measurement of Na+-dependent phosphate uptake.
  • Use of ion gradients and membrane-permeable anions.
  • Incubation with polyclonal antibodies against duodenal, jejunal, and ileal membrane vesicles.

    • Main Results:

    • Phosphate uptake is localized to the brush-border of duodenal enterocytes and is Na+-dependent.
    • Lithium (Li+) can substitute for sodium (Na+).
    • Phosphate transport is stimulated by intravesicular K+, Cs+, Rb+, or choline and requires membrane-permeable anions.
    • The phosphate carrier mechanism is electrogenic, likely involving the cotransport of two Na+ with one H2PO4-.
    • Antibodies against jejunal and ileal vesicles activated phosphate transport, whereas anti-duodenal antibodies inhibited it.

    Conclusions:

    • The phosphate transport mechanism in the rabbit small intestine is electrogenic and involves cotransport with Na+ and H+.
    • The affinity of the carrier for H2PO4- is pH-dependent.
    • Regional differences in antibody-mediated effects suggest distinct antibody targets or regulatory mechanisms in different parts of the small intestine.