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Related Experiment Videos

Cellular morphology and distribution on a stretching blood-material interface.

C R McMillin1, M R Malladi, D W Ott

  • 1Department of Biomedical Engineering, University of Akron, Ohio 44325.

Journal of Biomedical Materials Research
|April 1, 1988
PubMed
Summary

Mechanical stretching of blood-contacting surfaces, like Silastic tubing, can negatively impact blood compatibility. Increased stretching and contact time promote platelet aggregation and white blood cell adhesion, indicating adverse effects.

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Area of Science:

  • Biomaterials Science
  • Hemocompatibility Research
  • Surface Science

Background:

  • Understanding cellular and protein interactions with deforming biomaterials is crucial for developing safer medical devices.
  • Blood-contacting surfaces in medical devices can undergo mechanical stress, potentially altering their hemocompatibility.

Purpose of the Study:

  • To investigate the effects of mechanical deformation (stretching) on the hemocompatibility of Silastic tubing.
  • To analyze the interactions of blood cellular elements and proteins with fatigued blood contact surfaces.

Main Methods:

  • Ex vivo canine arteriovenous shunt experiments were performed using Silastic tubing.
  • Tubing segments were subjected to controlled stretching (20-60% strain) at varying frequencies and durations.

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  • Surface analysis was conducted using scanning electron microscopy and interference phase contrast microscopy.

    • Main Results:

    • Stretching induced increased platelet pseudopod extension and aggregation compared to unstretched controls.
    • Higher strain and longer blood contact times led to nonuniform platelet aggregations and fibrin formation.
    • Significant white blood cell adhesion, including granulocytes, was observed on stretched surfaces, indicating reduced hemocompatibility.

    Conclusions:

    • Substrate stretching of Silastic tubing adversely affects its blood compatibility.
    • Mechanical fatigue of biomaterials can promote prothrombotic and inflammatory responses.
    • Findings highlight the importance of considering mechanical properties in the design of blood-contacting medical devices.