Postprandial lipemia causes oxidative stress in dogs

Area of Science:

• Veterinary Medicine
• Biochemistry
• Canine Health

Background:

• Oxidative stress (OS) is linked to postprandial lipemia (PPL) in humans, but its effects in dogs require further study.
• Lipemia can interfere with spectrophotometric assays used to measure OS parameters.

Purpose of the Study:

• To investigate the impact of PPL on OS parameters in healthy dogs.
• To differentiate between in vivo PPL and in vitro simulated lipemia effects on OS markers.

Main Methods:

• Compared lipemic postprandial samples with non-lipemic samples in twenty dogs.
• Simulated PPL in vitro using lipid emulsion and canine serum.
• Assessed OS parameters including uric acid, albumin, total bilirubin, total antioxidant capacity (TAC), total oxidant capacity (TOC), and lipid peroxidation.

Main Results:

• In vivo PPL increased albumin, TAC-CUPRAC, TAC-FRAP, uric acid, TOC, and total bilirubin, while decreasing TAC-ABTS. Lipid peroxidation remained unchanged.
• In vitro lipemia increased albumin and uric acid at various concentrations, and total bilirubin at the highest concentration. TAC-ABTS and TAC-FRAP increased, but TAC-CUPRAC, TOC, and lipid peroxidation were unaffected.

Conclusions:

• Both in vivo PPL and in vitro lipemia influence canine OS markers, with effects varying by marker.
• The postprandial state and lipemic samples should be avoided in canine OS assessments to ensure accurate results.